The store said all proceeds will go to the Family Equality Council, a group working to defeat a gay marriage ban in Minnesota.
Voters there will decide this November whether to put the ban into the state constitution.
A spokesman for Minnesota For Marriage said Target is insulting a majority of their customers by selling the t-shirts.
Target fired back saying they're pleased to be able to sell the shirts, while also helping support the LGBT community.
The t-shirts are being sold online through the end of the month.
This controversy comes two years after the retailer angered gay marriage supporters by making a political donation to a gay marriage opponent running for governor’s office in Minnesota.
The shirts can be seen by clicking this link.